{"product_id":"biolegend-606101","title":"Biolegend, 606101, Purified anti-Cytokeratin 6 Antibody, 25μg","description":"\u003cp\u003eKeratins are main structural proteins of epithelial cells and form a cytoplasmic intermediate filament network, which is required for cell integrity and protecting cells from stress or injury. Keratin 6 (KRT6), also known as cytokeratin-6 or CK-6, is a type II intermediate filament protein constitutively expressed in all major types of epithelial appendages, such as hair, nail, and glands. Expression of KRT6 is induced by cell injury, infection, and disease. KRT6 has been identified as a marker of mammary gland progenitor cells. Defects in KRT6 genes have been associated with the inherited disorder pachyonychia congenital (PC), leading to structural abnormalities of nail, palms, and soles.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human cytokeratin 6A peptide (2-18 a.a.) conjugated to KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/ml (1:500-1:5000 dilution). For immunocytochemistry, a concentration range of 0.1 - 1.0 µg\/ml (1:500-1:5000 dilution) is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For immunohistochemistry, the suggested antigen retrieval buffer is either Tris-EDTA or sodium citrate (Cat. No. 420901).\u003cbr\u003e\nProduct Citations: Koliakou E, et al. 2022. Int J Mol Sci. 23: . PubMed\u003cbr\u003e\nRRID: AB_2734500 (BioLegend Cat. No. 606101) AB_2734501 (BioLegend Cat. No. 606102)\u003cbr\u003e\nStructure: 564 amino acids with a predicted molecular weight of 60 kD. Type II intermediate filament.\u003cbr\u003e\nDistribution: Cytoplasm\u003cbr\u003e\nFunction: KRT6 is a type II intermediate filament protein required for cell integrity and protecting cells from stress or injury.\u003cbr\u003e\nInteraction: KRT6 interacts with KRT16, KRT17, and TCHP.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: Arumugam A, et al. 2014. Biochem. Biophys. Res. Commun. 451:394. Cao L, et al. 2010. J. Appl. Toxicol. 30:416. Gu LH, Coulombe PA. 2008. Am. J. Pathol. 173:752. Wong P, Coulombe PA. 2003. J. Cell Biol. 163:327. Lin MT, et al. 1999. Exp. Dermatol. 8:115. Takahashi K, et al. 1995. J. Biol. Chem. 4:18581.\u003cbr\u003e\nGene ID: 3853\u003cbr\u003e\nUniProt: View information about Cytokeratin 6 on UniProt.org\u003cbr\u003e\nClone: W17006B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Keratin, type II cytoskeletal 6, Cytokeratin-6, CK-6, Keratin-6, KRT6\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866310004905,"sku":"606101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-606101","provider":"Iright","version":"1.0","type":"link"}