{"product_id":"biolegend-608456","title":"Biolegend, 608456, Alexa Fluor® 488 anti-RBFOX3 (NeuN) Antibody, 100μg","description":"\u003cp\u003eRBFox3, commonly referred to as NeuN, is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name RBFox is an acronym of \"Feminizing locus on X\". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. RBFox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The antibody reveals strong nuclear and distal cytoplasmic staining for RBFox3\/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This RBFox3\/NeuN antibody is therefore an excellent marker of neuronal cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full length recombinant human RBFOX3, isoform 5\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: IHC-F - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunohistochemistry in paraffin-embedded tissues, a concentration range of 2.0 - 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nAdditional Product Notes: For immunohistochemistry in paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) results in dim signal.\u003cbr\u003e\nRRID: AB_3106162 (BioLegend Cat. No. 608455) AB_3106162 (BioLegend Cat. No. 608456)\u003cbr\u003e\nStructure: RBFox3 is a 312 amino acid protein with a predicted molecular weight of 34 kD\u003cbr\u003e\nDistribution: Mature Neurons, Neural Stem Cells\/ Cytoplasm and Nucleus\u003cbr\u003e\nFunction: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nCell Type: Mature Neurons, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: Dhekne HS, et al. 2018. Elife. 7:e40202. Sun Y, et al. 2020. J Neuroinflammation. 17:72. Schwabenland M, et al. 2021. Immunity. 54:1594-1610.\u003cbr\u003e\nGene ID: 146713\u003cbr\u003e\nUniProt: View information about RBFOX3 on UniProt.org\u003cbr\u003e\nClone: W18230A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NeuN, RNA binding protein fox-1 homolog 3 (RBFox3), hexaribonucleotide binding protein 3, HRNBP3\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866337398953,"sku":"608456","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-608456","provider":"Iright","version":"1.0","type":"link"}