{"product_id":"biolegend-609052","title":"Biolegend, 609052, Purified anti-RORγt Antibody, 100μg","description":"\u003cp\u003eRORγt is a member of the nuclear hormone receptor family of transcription factors. RORγt is the isoform 2 of the RORC gene. It is produced by alternative splicing where the two 5’-exons are replaced by an alternative exon downstream in the gene, this splicing causes a shorter protein carrying three distinctive amino acids at its N-terminal end which are not present on RORγ. RORγt is mainly expressed on immature CD4+\/CD8+ thymocytes and Th17 cells. It is important on lymphoid organogenesis in lymph nodes and Peyer’s patches but not on spleen. It plays an important role in promoting differentiation of pro-inflammatory Th17 cells by reducing apoptosis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide from human RORγt\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICFC, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg\/mL. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody does not cross react with mouse RORγ or RORγt.\u003cbr\u003e\nRRID: AB_2927967 (BioLegend Cat. No. 609051) AB_2927967 (BioLegend Cat. No. 609052)\u003cbr\u003e\nStructure: RORγt is a 497 amino acid protein with a predicted molecular weight of 56 kD.\u003cbr\u003e\nDistribution: Expressed on different subsets of T cells (mature and immature), on thymus and lymph nodes. Also, it has been found expressed on Treg cells in colon.\u003cbr\u003e\nFunction: Promotion of pro-inflammatory Th17 cells by downregulation of Fas ligand and IL2 which reduce apoptosis.\u003cbr\u003e\nLigand\/Receptor: Ligand are cholesterol metabolites\u003cbr\u003e\nCell Type: Lymphocytes, Macrophages, NK cells, NKT cells, T cells, Th17, Tregs\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors\u003cbr\u003e\nMolecular Family: Innate Immune Signaling\u003cbr\u003e\nAntigen References: Castro G, et al. 2017. PloS One. 12: e0181868. Villey I, et al. 1999. Eur J Immunol. 29:4072-80. Lamb D, et al. 2021. Respir Res. 22:158.\u003cbr\u003e\nGene ID: 6097\u003cbr\u003e\nUniProt: View information about RORgammat on UniProt.org\u003cbr\u003e\nClone: A21005D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: retinoic acid receptor (RAR)-related orphan receptor gamma T, ROR gamma2, RAR Related Orphan Receptor C, TOR, Retinoic Acid-Binding Receptor Gamma, IMD42, NR1f3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866072895657,"sku":"609052","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-609052","provider":"Iright","version":"1.0","type":"link"}