{"product_id":"biolegend-612901","title":"Biolegend, 612901, Purified anti-DNA-PKcs Phospho (Thr2609) Antibody, 25μg","description":"\u003cp\u003eDNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70\/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Modified peptide\u003cbr\u003e\nFormulation: This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg\/ml.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Reindl J, et al. 2022. Sci Rep. 12:1305. PubMed Eid M, et al. 2014. J Immunol. 192:5529. PubMed\u003cbr\u003e\nRRID: AB_315784 (BioLegend Cat. No. 612901) AB_315785 (BioLegend Cat. No. 612902)\u003cbr\u003e\nStructure: PI3\/PI4-Kinases family. Spliced products 460 kD, 470 kD\u003cbr\u003e\nDistribution: Nuclear\u003cbr\u003e\nFunction: Serine\/Threonine kinase, dsDNA break repair, VDJ recombination, transcriptional modulator\u003cbr\u003e\nInteraction: Ku70\/Ku80, KIP, DNA-ligase IV, XRCC4\u003cbr\u003e\nModification: Phosphorylation\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, DNA Repair\/Replication, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Hartley K, et al. 1995. Cell. 82:849. 2. Connelly M, et al. 1996. Gene. 175:271. 3. Douglas P, et al. 2002. Biochem. J. 368:243 4. Calsou P, et al. 2003. J. Mol. Biol. 326:93.\u003cbr\u003e\nRegulation: Must bind DNA ends to become active\u003cbr\u003e\nGene ID: 5591\u003cbr\u003e\nUniProt: View information about DNA-PKcs Phosphorylated on UniProt.org\u003cbr\u003e\nClone: 10B1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860778111145,"sku":"612901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-612901","provider":"Iright","version":"1.0","type":"link"}