{"product_id":"biolegend-615002","title":"Biolegend, 615002, Purified anti-GSK-3α\/β Antibody, 100μg","description":"\u003cp\u003eGSK-3α\/β is a ubiquitously expressed serine\/threonine kinase that can phosphorylate a wide range of substrates. While GSK-3 was initially shown to regulate energy metabolism through phosphorylation of glycogen synthase, it has been shown that the enzyme has a wide variety of substrates in vivo and regulates signaling pathways that control cell growth, development,  apoptosis, and oxidative stress responses. Each isoform if encoded by separate alleles; the isoforms show 66% homology.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant full-length GSK-3β\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone recognizes both a and ß isoforms of GSK-3 by both WB and IP.This clone failed to stain PFA-fixed HeLa cells that were permeabilized with methanol or Triton-X. We therefore do not recommend using this clone for ICC.\u003cbr\u003e\nRRID: AB_2814452 (BioLegend Cat. No. 615001) AB_2814453 (BioLegend Cat. No. 615002)\u003cbr\u003e\nStructure: GSK-3α is a 483 amino acid protein with a predicted molecular weight of 51 kD; GSK-3β is a 420 amino acid protein with a predicted molecular weight of 47 kD.\u003cbr\u003e\nDistribution: Both isoforms ubiquitously expressed GSK-3α: cytosol; GSK-3β: nucleus\u003cbr\u003e\nFunction: Cell Signaling\u003cbr\u003e\nAntigen References: Imazu M, et al. 1984. J. Biol. Chem. 259: 1813. Cross DA, et al. 1995. Nature. 378: 785. Kazi A, et al. 2018. Nat. Commun. 9: 5154.\u003cbr\u003e\nGene ID: 29312932\u003cbr\u003e\nUniProt: View information about GSK-3alpha\/beta on UniProt.org\u003cbr\u003e\nClone: W17173C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Glycogen Synthase Kinase, Glycogen Synthase Kinase 3 Alpha, Glycogen Synthase Kinase Beta, GSK3A, GSK3B\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866314789033,"sku":"615002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-615002","provider":"Iright","version":"1.0","type":"link"}