{"product_id":"biolegend-616001","title":"Biolegend, 616001, Purified anti-LEF1 Antibody, 25μg","description":"\u003cp\u003eLymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF\/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg\/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: W17155A recognizes multiple isoforms of LEF1.When tested for ICC, W17155A failed to stain PFA-fixed Jurkat cells permeabilized with methanol. We recommend Triton X-100 permeabilization.\u003cbr\u003e\nRRID: AB_2814460 (BioLegend Cat. No. 616001) AB_2814461 (BioLegend Cat. No. 616002)\u003cbr\u003e\nStructure: LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.\u003cbr\u003e\nDistribution: Lymphoid tissue enriched\/Nuclear localization\u003cbr\u003e\nFunction: Canonical Wnt signaling\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers, TCRs\u003cbr\u003e\nAntigen References: 1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184. 2. Waterman ML, et al. 1991. Genes Dev. 5:656. 3. Hovanes K, et al. 2001. Nat Genet. 28:53. 4. Bruhn L, et al. 1997. Genes Dev. 11:640. 5. Nguyen A, et al. 2005. Int. J. Oncol. 27:949. 6. Petropoulos K, et al. 2008. J. Exp. Med. 205:515.\u003cbr\u003e\nGene ID: 51176\u003cbr\u003e\nUniProt: View information about LEF1 on UniProt.org\u003cbr\u003e\nClone: W17155A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphoid enhancer-binding factor 1, TCF1α, TCF10\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866314494121,"sku":"616001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-616001","provider":"Iright","version":"1.0","type":"link"}