{"product_id":"biolegend-616702","title":"Biolegend, 616702, Purified anti-NF-κB p50 Antibody, 100μg","description":"\u003cp\u003eNF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel\/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: recombinant full-length human NF-κB p50\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg\/ml.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 2.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.\u003cbr\u003e\nProduct Citations: Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed Kao C, et al. 2008. J Biol Chem. 283:15309. PubMed Boudesco C, et al. 2018. Blood. 132:510. PubMed Huang F, et al. 2007. J Immunol. 179:6504. PubMed\u003cbr\u003e\nRRID: AB_315855 (BioLegend Cat. No. 616701) AB_315856 (BioLegend Cat. No. 616702)\u003cbr\u003e\nStructure: Member of the Rel\/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.\u003cbr\u003e\nDistribution: Ubiquitously expressed\u003cbr\u003e\nFunction: A transcription factor, originally identified as an activator of kappa light chain in B cells\u003cbr\u003e\nInteraction: Interacts with NF-κB p65 and IκB\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review). 2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).\u003cbr\u003e\nRegulation: NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.\u003cbr\u003e\nGene ID: 4790\u003cbr\u003e\nUniProt: View information about NF-kappaB p50 on UniProt.org\u003cbr\u003e\nClone: 4D1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862413955241,"sku":"616702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-616702","provider":"Iright","version":"1.0","type":"link"}