{"product_id":"biolegend-621052","title":"Biolegend, 621052, Purified anti-LEF1 Antibody, 100μg","description":"\u003cp\u003eLymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF\/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream gene expression. LEF1 is implicated as a transcriptional regulator in the transformation of cancer cells in various diseases such as colonic adenocarcinoma. Due to its role in Wnt\/β-catenin signaling pathway, LEF1 has been indicated as a facilitator of EMT and potential biomarker for various cancers.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: LEF-1 is known to have 7 isoforms. WB (Western blotting) or IHC-P (Immunohistochemistry FFPE) is not suggested for this product. For WB, IP (immunoprecipitation) or ICC (immunocytochemistry) applications, please refer to clones 15H5A18 (Cat. No. 653101\/653102) or W17155A (Cat. No. 616001\/616002). For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization.\u003cbr\u003e\nRRID: AB_3083406 (BioLegend Cat. No. 621051) AB_3083406 (BioLegend Cat. No. 621052)\u003cbr\u003e\nStructure: LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.\u003cbr\u003e\nDistribution: Lymphoid tissue\/Nuclear localization\u003cbr\u003e\nFunction: Canonical Wnt signaling\u003cbr\u003e\nInteraction: Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3\u003cbr\u003e\nLigand\/Receptor: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers, TCRs\u003cbr\u003e\nAntigen References: Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184-95. Waterman ML, et al. 1991. Genes Dev. 5:656-69. Hovanes K, et al. 2001. Nat Genet. 28:53-7.. Bruhn L, et al. 1997. Genes Dev. 11:640-53. Nguyen A, et al. 2005. Int J Oncol. 27:949-56. Petropoulos K, et al. 2008. J Exp Med. 205:515-22.\u003cbr\u003e\nGene ID: 51176\u003cbr\u003e\nUniProt: View information about LEF1 on UniProt.org\u003cbr\u003e\nClone: W17021C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha), TCF1α, TCF10, TCF7L3\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866310529193,"sku":"621052","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-621052","provider":"Iright","version":"1.0","type":"link"}