{"product_id":"biolegend-621702","title":"Biolegend, 621702, Purified anti-STIM1 Antibody, 100μg","description":"\u003cp\u003eStromal Interaction Molecule 1, or STIM1 is a single pass transmembrane, resident ER protein that facilitates extracellular calcium uptake following the depletion of intracellular stores. STIM1 directly senses loss of ER-lumenal calcium via an EF hand domain; elevated ER calcium levels stimulate redistribution of STIM1 to close proximity to the plasma mebrane, where it activates ORAI1, a pore component of the store-operated calcium entry system.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human STIM1 protein corresponding to amino acid residues 436-685\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: When testing W18094A testing for ICC, we observed poor staining of PFA-fixed cells that were permeabilized with methanol.When testing W18094A for WB, a minor non-specific band of ~50 kD was observed by WB at higher antibody concentrations.\u003cbr\u003e\nRRID: AB_2814470 (BioLegend Cat. No. 621701) AB_2814471 (BioLegend Cat. No. 621702)\u003cbr\u003e\nStructure: STIM1 is a 685 amino acid protein with a predicted molecular weight of 77 kD.\u003cbr\u003e\nDistribution: ER Membrane\/Ubiquitously expressed\u003cbr\u003e\nFunction: Calcium sensing and uptake\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Innate Immune Signaling, Ion Channels\u003cbr\u003e\nAntigen References: Roos J, et al. 2005. J. Cell Biol. 169:435. Peinelt C, et al. 2006. Nat Cell Biol. 8:771. Baba Y, et al. 2006. Proc Natl Acad Sci USA. 103:16704.\u003cbr\u003e\nGene ID: 6786\u003cbr\u003e\nUniProt: View information about STIM1 on UniProt.org\u003cbr\u003e\nClone: W18094A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Stromal Interaction Molecule 1, GOK, IMD10, TAM1, STRMK\u003cbr\u003e\nIsotype: Rat IgG2b, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866333040809,"sku":"621702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-621702","provider":"Iright","version":"1.0","type":"link"}