{"product_id":"biolegend-625001","title":"Biolegend, 625001, Purified anti-SLP-76 Antibody, 50microl","description":"\u003cp\u003eSLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S\/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76\/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.\u003cbr\u003e\n50microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: amino acids 216-434 of human SLP-76\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, aliquot and store frozen at -20°C. Avoid freeze-thaw cycles.\u003cbr\u003e\nApplication: WB - Quality tested IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: The 200 µL size can be used for approximately 40 Western blots.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Deford-Watts LM, et al. 2011. J. Immunol. 186:6839. PubMed.\u003cbr\u003e\nProduct Citations: Bacchelli C, et al. 2017. J Allergy Clin Immunol. 139:634. PubMed DeFord-Watts L, et al. 2011. J Immunol. 186:6839. PubMed\u003cbr\u003e\nRRID: AB_2136888 (BioLegend Cat. No. 625002)\u003cbr\u003e\nStructure: Phosphoprotein 76 kD containing SH2 domain in the C-terminus\u003cbr\u003e\nDistribution: Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.\u003cbr\u003e\nFunction: Adaptor molecule involved in B cell receptor and T cell receptor signaling\u003cbr\u003e\nInteraction: Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2\u003cbr\u003e\nModification: Phosphorylation (Y113, Y128, Y145, Y423, Y426)\u003cbr\u003e\nCell Type: B cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Jackman KJ, et al. 1995. J. Biol. Chem. 270:7029. 2. Wardenberg JB, et al. 1996. J. Biol. Chem. 271:19641. 3. Tuosto L, et al. 1996. J. Exp. Med. 184:1161. 4. Motto DG, et al. 1996. J. Exp. Med. 183:1937.\u003cbr\u003e\nGene ID: 3937\u003cbr\u003e\nUniProt: View information about SLP-76 on UniProt.org\u003cbr\u003e\nClone: H76\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866453299369,"sku":"625001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-625001","provider":"Iright","version":"1.0","type":"link"}