{"product_id":"biolegend-626454","title":"Biolegend, 626454, Alexa Fluor® 647 anti-PTEN Phospho (Ser380) Antibody, 100μg","description":"\u003cp\u003ePTEN is a tumor suppressor gene which encodes a phosphatase that preferentially dephosphorylates phosphoinositide substrates and thereby modulates cell cycle progression and cell survival. PTEN is an important negative regulator of the PI3K\/AKT\/mTOR signaling pathway by dephosphorylating PIP3. PTEN is commonly mutated in human cancer, hence, loss of expression\/function of PTEN due to mutations have been observed in prostate cancer, glioblastoma, endometrial, lung and breast cancers. Other non-cancerous clinical disorders associated with PTEN mutation are Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and proteus-like syndrome. PTEN is phosphorylated at several residues and some lead to increase in activity while others lead to loss of function and degradation. Specifically, phosphorylation at serine 380 residue by casein kinase 2 (CK2), leads to degradation of PTEN in the proteasome.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide from human PTEN phosphorylated at Serine 280\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: For use of this antibody (clone A22005D) in intracellular staining in flow cytometry (ICFC), we recommend using True-Phos ™ Perm Buffer (Cat. No. 425401) for fixation\/permeabilization. For use of this antibody (clone A22005D) in immunohistochemistry on FFPE tissues (IHC-P), we recommend antigen retrieval with Tris-EDTA pH 9.0 Buffer (Cat. No. 422703).\u003cbr\u003e\nRRID: AB_3662411 (BioLegend Cat. No. 626453)\u003cbr\u003e\nStructure: PTEN is a 403 amino acid protein with a predicted molecular weight of 55 kD. It has a tyrosine phosphatase domain and a C2 domain\u003cbr\u003e\nDistribution: Cytoplasmic (Inner cellular membrane) and nuclear\u003cbr\u003e\nFunction: PTEN functions as a lipid and protein phosphatase. It is negatively regulated by phosphorylation at the C-terminal domain which leads to proteasomal degradation.\u003cbr\u003e\nInteraction: P53, PTK2, MAGI3, CSNK2A2, NEDD4, NR3C4, MVP\u003cbr\u003e\nBiology Area: Angiogenesis, Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Dempsey DR, et al. 2021. Nature Structural \u0026amp; Molecular Biology. 28:858-868. Gupta A, et al. 2016. JBC. 291:18465-73. Song MS, et al. 2012. Nature Reviews Molecular Cell Biology. 13:283-96. Vazquez F, et al. 2000. Mol Cell Biol. 20:5010-8.\u003cbr\u003e\nGene ID: 5728\u003cbr\u003e\nUniProt: View information about PTEN Phospho Ser380 on UniProt.org\u003cbr\u003e\nClone: A22005D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Phosphatase and tensin homolog, PTEN1, BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866075779241,"sku":"626454","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-626454","provider":"Iright","version":"1.0","type":"link"}