{"product_id":"biolegend-627203","title":"Biolegend, 627203, PE anti-TRIM Antibody, 25tests","description":"\u003cp\u003eTRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial recombinant human TRIM (amino acids 29-186)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, ELISA1, and immunofluorescence microscopy2.Clone TRIM-4 has been shown to cross-react to mouse TRIM1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Huynh T, et al. 2001. Scand. J. Immunol. 54:146.Kirchgessner H, et al. 2001. J. Exp. Med. 193:1269.\u003cbr\u003e\nRRID: AB_2565761 (BioLegend Cat. No. 627203) AB_2565762 (BioLegend Cat. No. 627204)\u003cbr\u003e\nStructure: Type III membrane protein, 30 kD disulfide-linked dimeric phosphoprotein\u003cbr\u003e\nDistribution: T cells, lower expression levels in NK cells\u003cbr\u003e\nFunction: Links extracellular and intracellular signaling pathways in T cell activation\u003cbr\u003e\nInteraction: PI3K regulatory subunit α, TCRα, β, and ζ.\u003cbr\u003e\nModification: Tyrosine phosphorylation by Src kinases\u003cbr\u003e\nCell Type: T cells, NK cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Bruyns E, et al. 1998. J. Exp. Med. 188:561.2. Hubener C, et al. 2000. Immunogenetics 51:154.\u003cbr\u003e\nGene ID: 50852\u003cbr\u003e\nUniProt: View information about TRIM on UniProt.org\u003cbr\u003e\nClone: TRIM-4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell receptor-interacting molecule\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866471813289,"sku":"627203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-627203","provider":"Iright","version":"1.0","type":"link"}