{"product_id":"biolegend-631751","title":"Biolegend, 631751, Purified anti-Profilin-1 Antibody, 25μg","description":"\u003cp\u003eProfilin-1 is an actin-binding protein responsible for the regulation of actin dynamics. Profilin-1 assists in the exchange of ADP for ATP on G-actin, facilitating the polymerization of actin monomers into filaments by promoting assembly at the growing end. Thus, profilin-1 is implicated in processes like cell motility, cytokinesis, and endocytosis, highlighting its significance in maintaining cellular structure and function. Profilin-1 also interacts with numerous proteins involved in signaling pathways, influencing cellular responses to intracellular and extracellular cues. Emerging evidence also shows that profilin-1 can bind to microtubules, facilitating interactions between the two major components of the cytoskeleton. Mutations in profilin-1 have been associated with amyotrophic lateral sclerosis (ALS) and is suspected to cause insoluble aggregates due to disruption in axonal outgrowth, cell integrity and protein distribution.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant fragment of the N terminus of human Profilin 1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody (clone A18224E) is not suitable for immunocytochemistry (ICC). For immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). Antigen retrieval with Sodium Citrate is not recommended as it results in mis-localized signal.\u003cbr\u003e\nRRID: AB_3097566 (BioLegend Cat. No. 631751) AB_3097566 (BioLegend Cat. No. 631752)\u003cbr\u003e\nStructure: Profilin-1 is an 140 amino-acid protein with a predicted molecular weight of 15 kD.\u003cbr\u003e\nDistribution: Ubiquitous, cytoskeleton\/cytoplasm\u003cbr\u003e\nFunction: Actin dynamics, cell shape and motility\u003cbr\u003e\nInteraction: Actin, Ena\/VASP proteins, phosphoinositides, ROCK, RAP1, CDC42\u003cbr\u003e\nLigand\/Receptor: Profilin-1 binds actin monomers\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Cell Structure\u003cbr\u003e\nMolecular Family: Microfilaments\u003cbr\u003e\nAntigen References: Murk K, et al. 2021. Front Cell Dev Biol. 9:681122. Karlsson R, et al. 2021. J Cell Physiol. 236:7256-7265. Davey RJ, et al. 2020. Biophys Rev. 12:827-849.\u003cbr\u003e\nGene ID: 5216\u003cbr\u003e\nUniProt: View information about Profilin-1 on UniProt.org\u003cbr\u003e\nClone: A18224E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Profilin 1, PFN1, Profilin1, Epididymis tissue protein Li 184a\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866063949993,"sku":"631751","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-631751","provider":"Iright","version":"1.0","type":"link"}