{"product_id":"biolegend-632556","title":"Biolegend, 632556, PE anti-CREB1 Phospho (Ser133) Antibody, 100tests","description":"\u003cp\u003eCyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+\/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen\/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions. Phosphorylation of CREB1 at Ser133 promotes the recruitment of co-activator proteins CBP and p300, which has been proposed to increase transcription of CREB-dependent genes. Ser133 can also be phosphorylated by other proteins such as MSK1\/2 in response to the activation of MAPK signaling.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide for human CREB1 phosphorylated at serine 133\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization with True-Phos™ Perm buffer (Cat. No. 425401).\u003cbr\u003e\nRRID: AB_3662315 (BioLegend Cat. No. 632555) AB_3662315 (BioLegend Cat. No. 632556)\u003cbr\u003e\nStructure: CREB1 contains 327 amino acids at a predicted molecular weight around 37 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Nucleus\u003cbr\u003e\nFunction: Under physiological conditions, CREB1 maintains cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving a neuroprotective role. Under pathological conditions, increased levels of CREB1 is associated with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.\u003cbr\u003e\nInteraction: CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nAntigen References: Steven A, et al. 2020. Cell Mol Life Sci. 77:4049-4067. Lin PH, et al. 2020. Diagnostics (Basel). 10:295. Sakamoto K, et al. 2011. J Neurochem. 116:1-9. Wen AY, et al. 2010. J Immunol. 185:6413-9. Arnould T, et al. 2002. EMBO J. 21:53-63. Naqvi S, et al. 2014. Biochem J. 458:469-79.\u003cbr\u003e\nGene ID: 1385\u003cbr\u003e\nUniProt: View information about CREB1 on UniProt.org\u003cbr\u003e\nClone: A22026B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CAMP responsive element binding protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein; CAMP-Responsive Element-Binding Protein 1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866076827817,"sku":"632556","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-632556","provider":"Iright","version":"1.0","type":"link"}