{"product_id":"biolegend-641401","title":"Biolegend, 641401, Purified anti-c-Fos Antibody, 25microl","description":"\u003cp\u003eThe c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1.\u003cbr\u003e\n25microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.\u003cbr\u003e\nPreparation: The antibody was purified by antigen-affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store at -20°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 - 1:4000 antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product may contain other non-IgG subtypes. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fan W, et al. 2009. Brain Res Red. 59:324. PubMed Sugimoto K, et al. 2014. Neurosci Res. PubMed\u003cbr\u003e\nProduct Citations: Cong J, et al. 2022. Theranostics. 12:5204. PubMed Chung H, et al. 2021. Nat Methods. 18:1204. PubMed Sugimoto K, et al. 2015. Neurosci Res. 96: 20-29. PubMed Fan W, et al. 2009. Brain Res Rev. 59:324. PubMed\u003cbr\u003e\nRRID: AB_2106632 (BioLegend Cat. No. 641401) AB_2106629 (BioLegend Cat. No. 641402)\u003cbr\u003e\nStructure: c-fos heterodimerizes with c-jun to form the transcription factor AP-1; contains a bZIP region with a DNA-binding domain and a leucine zipper domain; approximately 62 kD.\u003cbr\u003e\nDistribution: Expressed in the nucleus of many tissues\/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes.\u003cbr\u003e\nFunction: FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal form\u003cbr\u003e\nInteraction: Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1\u003cbr\u003e\nModification: Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Neuroscience, Synaptic Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Dony C, et al. 1987. Nature 328:711. 2. Grigoriadis AE, et al. 1994. Science 266:443. 3. Glover JNM, et al. 1995. Nature 373:257.\u003cbr\u003e\nRegulation: c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia.\u003cbr\u003e\nGene ID: 2353\u003cbr\u003e\nUniProt: View information about c-Fos on UniProt.org\u003cbr\u003e\nClone: Poly6414\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866471223465,"sku":"641401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-641401","provider":"Iright","version":"1.0","type":"link"}