{"product_id":"biolegend-644838","title":"Biolegend, 644838, KIRAVIA Blue 520™ anti-T-bet Antibody, 100μg","description":"\u003cp\u003eT-bet, also known as T-box transcription factor T-bet, is considered to be a \"master regulator\" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix\/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.\u003cbr\u003e\nProduct Citations: Reilly NA, et al. 2024. iScience. 109496:27. PubMed\u003cbr\u003e\nRRID: AB_2888710 (BioLegend Cat. No. 644837) AB_2888710 (BioLegend Cat. No. 644838)\u003cbr\u003e\nStructure: T-box transcription factor, approximately 58 kD.\u003cbr\u003e\nDistribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.\u003cbr\u003e\nFunction: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.\u003cbr\u003e\nGene ID: 30009\u003cbr\u003e\nUniProt: View information about T-bet on UniProt.org\u003cbr\u003e\nClone: 4B10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-box expressed in T cells, T box 21, TBLYM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862408417449,"sku":"644838","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-644838","provider":"Iright","version":"1.0","type":"link"}