{"product_id":"biolegend-645806","title":"Biolegend, 645806, PE anti-p53 Antibody, 100tests","description":"\u003cp\u003ep53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and\/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300\/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: p53 N-terminal linear epitope aa 20-25\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Vojtesek B, et al. 1992. J. Immunol. Methods 151:237. Stephen CW, et al. 1995. J. Mol. Biol. 248:58.\u003cbr\u003e\nProduct Citations: Padalino G, et al. 2021. Eur J Med Chem. 226:113823. PubMed Catenacci D, et al. 2015. Gastroenterology. 149: 1794-1803.e4. PubMed\u003cbr\u003e\nRRID: AB_2562267 (BioLegend Cat. No. 645805) AB_2562268 (BioLegend Cat. No. 645806)\u003cbr\u003e\nStructure: Tetramer; 53 kD\u003cbr\u003e\nDistribution: Nuclear when activated\u003cbr\u003e\nFunction: Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity\u003cbr\u003e\nInteraction: mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300\/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73\u003cbr\u003e\nCell Type: Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication, Stem Cells\u003cbr\u003e\nGene ID: 7157\u003cbr\u003e\nUniProt: View information about p53 on UniProt.org\u003cbr\u003e\nClone: DO-7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor protein p53 (TP53)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863013413033,"sku":"645806","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-645806","provider":"Iright","version":"1.0","type":"link"}