{"product_id":"biolegend-647401","title":"Biolegend, 647401, Purified anti-mMCP-8 Antibody, 25μg","description":"\u003cp\u003emMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell\/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant GST-mouse mMCP-8 fusion protein expressed in E.coli\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The optimal concentration should be determined by titration for each individual assay of interest.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ugajin T, et al. 2009. J. Leukoc. Biol. 86:1417. (IHC) Uto-Konomai A, et al. 2012. PLoS One. 7:e40343. PubMed Morshed M, et al. 2014. J Immunol. 192:5314. PubMed Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed Schwartz C, et al. 2014. PNAS. 111:5169. PubMed\u003cbr\u003e\nProduct Citations: Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed Morshed M, et al. 2014. J Immunol. 192:5314. PubMed Piliponsky AM, et al. 2019. Nat Immunol. 0.922916667. PubMed Webb LM, et al. 2019. J Exp Med. 216:1268. PubMed Monte L, et al. 2016. Cancer Res . 76: 1792-1803. PubMed Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed Imai Y, et al. 2017. Sci Rep. 10.1038\/s41598-017-10227-y. PubMed Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed\u003cbr\u003e\nRRID: AB_2069309 (BioLegend Cat. No. 647401) AB_2290790 (BioLegend Cat. No. 647402)\u003cbr\u003e\nStructure: A 247-aa polypeptide including a signal sequence of 18 aa followed by a 2-aa activation peptide (Gly-Glu) and a mature protease of 227 aa\u003cbr\u003e\nDistribution: mMCP-8 was initially isolated as a cDNA from a mouse mast cell line, but has recently been found to be expressed primarily by mouse basophils.\u003cbr\u003e\nCell Type: Basophils, Mast cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nAntigen References: 1. Lutzelschwab C, et al. 1998. Eur. J. Immunol. 28:1022. 2. Lunderius C, et al. 2001. Immunogenetics 53:225.\u003cbr\u003e\nGene ID: 4179\u003cbr\u003e\nUniProt: View information about mMCP-8 on UniProt.org\u003cbr\u003e\nClone: TUG8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Mouse Mast Cell Protease 8, Mcpt8\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864050585769,"sku":"647401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-647401","provider":"Iright","version":"1.0","type":"link"}