{"product_id":"biolegend-647504","title":"Biolegend, 647504, PE anti-XBP-1s Antibody, 100tests","description":"\u003cp\u003eXBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: XBP-1s recombinant protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print] Wang L, et al. 2011. Hum Mol Genet. PubMed Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed Shirley CM, et al. 2011 Blood. 117:6297. PubMed Vidal RL., et al. 2012. Hum Mol Genet. PubMed Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed Bonetti P, et al. 2013. Blood. 122:2233. PubMed Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC) Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed\u003cbr\u003e\nProduct Citations: Katsuyama E, et al. 2024. Nat Commun. 8304:15. PubMed Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed\u003cbr\u003e\nRRID: AB_2563447 (BioLegend Cat. No. 647503) AB_2563448 (BioLegend Cat. No. 647504)\u003cbr\u003e\nDistribution: Ubiquitously expressed.\u003cbr\u003e\nFunction: Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nAntigen References: 1. Liou HC, et al. 1990. Science 247:1581. 2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074. 3. Takahashi S, et al. 2002. Prostate 50:154.\u003cbr\u003e\nGene ID: 7494\u003cbr\u003e\nUniProt: View information about XBP-1s on UniProt.org\u003cbr\u003e\nClone: 143F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: X-box binding protein 1\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860803014825,"sku":"647504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-647504","provider":"Iright","version":"1.0","type":"link"}