{"product_id":"biolegend-648005","title":"Biolegend, 648005, Direct-Blot™ HRP anti-Hsp70 Antibody, 100microl","description":"\u003cp\u003eHsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Hsp70 protein from HeLa cells.\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nRRID: AB_2566707 (BioLegend Cat. No. 648005)\u003cbr\u003e\nStructure: Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate\u003cbr\u003e\nDistribution: Ubiquitous, cytoplasmic and nuclear\u003cbr\u003e\nFunction: Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance\u003cbr\u003e\nAntigen References: 1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571. 2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425. 3. Bukau B, et al. 1998. Cell 92:351.\u003cbr\u003e\nGene ID: 3308\u003cbr\u003e\nUniProt: View information about Hsp70 on UniProt.org\u003cbr\u003e\nClone: W27\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Heat shock protein 70\u003cbr\u003e\nIsotype: Mouse IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864085024937,"sku":"648005","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-648005","provider":"Iright","version":"1.0","type":"link"}