{"product_id":"biolegend-648870","title":"Biolegend, 648870, Purified anti-Fibronectin Antibody, 25μg","description":"\u003cp\u003eFibronectin is a glycoprotein that plays a critical role in cell adhesion, growth, migration, and differentiation. It is a major component of the extracellular matrix (ECM), where it helps to organize other matrix proteins and anchor cells via integrin receptors. Fibronectin is essential during embryonic development and wound healing, facilitating tissue morphogenesis and repair by guiding fibroblast and endothelial cell movement. It also contributes to mechanotransduction and regulates cellular signaling pathways involved in survival and proliferation. Dysregulation of fibronectin expression or structure has been implicated in fibrosis, cancer metastasis, and chronic inflammation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human plasma fibronectin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix\/permeabilize with any of the following: -Cyto-Fast™ Fix\/Perm Buffer Set (Cat. No. 426803) -True-Phos™ Perm Buffer (Cat. No. 425401) -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)\u003cbr\u003e\nRRID: AB_3720599 (BioLegend Cat. No. 648870) AB_3720599 (BioLegend Cat. No. 648871)\u003cbr\u003e\nStructure: Fibronectin is a 2477 amino acid protein with a predicted molecular weight of 272.3 kD.\u003cbr\u003e\nDistribution: Secreted, extracellular space\u003cbr\u003e\nFunction: Adhesion molecule\u003cbr\u003e\nInteraction: Collagen, Fibrin, heparin domains, Tenascin-C, Laminin, degraded by matrix metalloproteinases\u003cbr\u003e\nLigand\/Receptor: Integrin receptors, syndecans\u003cbr\u003e\nCell Type: Fibroblasts, Mesenchymal cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion\u003cbr\u003e\nMolecular Family: Adhesion Molecules\u003cbr\u003e\nAntigen References: Singh P, et al. 2010. Ann Rev Cell Dev. Biol. 26:397-419. Patten J, Wang K. 2021. Adv Drug Deliv Rev. 70:353-368. Sun Y, et al. 2025. J Cell Sci. 138(6):jcs263834.\u003cbr\u003e\nGene ID: 2335\u003cbr\u003e\nUniProt: View information about FN-1 on UniProt.org\u003cbr\u003e\nClone: C6F10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FN1, FN\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862950727849,"sku":"648870","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-648870","provider":"Iright","version":"1.0","type":"link"}