{"product_id":"biolegend-648874","title":"Biolegend, 648874, Purified anti-ZO-1 Antibody, 25μg","description":"\u003cp\u003eZonula occludens-1 (ZO-1) is a scaffolding protein critical for the assembly and maintenance of tight junctions in epithelial and endothelial cells. It links transmembrane tight junction proteins such as claudins and occludin to the actin cytoskeleton, thereby maintaining cell polarity and barrier integrity. ZO-1 also participates in signal transduction pathways that regulate cell proliferation and differentiation. Loss or mislocalization of ZO-1 is associated with impaired barrier function and has been implicated in various pathological conditions, including cancer and inflammatory diseases. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: Human HAP1 Knockout Cell Line – TJP1 (Horizon Cat. No. HZGHC006271c003)\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant protein corresponding to a central portion of human ZO-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICC, WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg\/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only\u003cbr\u003e\nRRID: AB_3720606 (BioLegend Cat. No. 648874) AB_3720606 (BioLegend Cat. No. 648875)\u003cbr\u003e\nStructure: ZO-1 is a 1748 amino acid protein with a predicted molecular weight of 195.5 kD.\u003cbr\u003e\nDistribution: Cell surface, tight junctions\u003cbr\u003e\nFunction: Scaffold protein\u003cbr\u003e\nInteraction: Claudins (CLDN1-5), Occludin (OCLN), actin\u003cbr\u003e\nCell Type: Endothelial cells, Epithelial cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Signal Transduction\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Cytoskeletal Proteins\u003cbr\u003e\nAntigen References: Kuo W, et al. 2022. Ann N Y Acad Sci. 1514(1):21-33. Van Itallie CM, et al. 2009. Mol Biol Cell. 20(17):3930-3940. Tornavaca O, et al. 2015. J Cell Biol. 208(6):821-838. Stevenson BR, et al. 1986. J Cell Biol. 103(3):755-66.\u003cbr\u003e\nGene ID: 7082\u003cbr\u003e\nUniProt: View information about ZO-1 on UniProt.org\u003cbr\u003e\nClone: W24004C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: tight junction protein 1 (TJP1), zona occludens protein 1 (ZO1)\u003cbr\u003e\nIsotype: Rat IgG2b, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866440028329,"sku":"648874","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-648874","provider":"Iright","version":"1.0","type":"link"}