{"product_id":"biolegend-651007","title":"Biolegend, 651007, Alexa Fluor® 647 anti-STAT3 Phospho (Tyr705) Antibody, 25tests","description":"\u003cp\u003eTyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KLH conjugated modified synthetic peptide\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.\u003cbr\u003e\nProduct Citations: Chen HY, et al. 2022. Elife. 11:. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed\u003cbr\u003e\nRRID: AB_2572085 (BioLegend Cat. No. 651007) AB_2572086 (BioLegend Cat. No. 651008)\u003cbr\u003e\nStructure: STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.\u003cbr\u003e\nDistribution: Ubiquitous.\u003cbr\u003e\nFunction: STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.\u003cbr\u003e\nCell Sources: Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: 1. Akira S, et al. 1994. Cell 77:63. 2. Zhang X, et al. 1995. Science 267:1990. 3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30. 4. Simon A, et al. 2000. Science 290:144. 5. Hoey T, et al. 1999. Adv. Immunol. 71:145.\u003cbr\u003e\nRegulation: The small GTPase Rac1 binds and regulates activity of STAT3.\u003cbr\u003e\nGene ID: 6774\u003cbr\u003e\nUniProt: View information about STAT3 Phospho Tyr705 on UniProt.org\u003cbr\u003e\nClone: 13A3-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860800852137,"sku":"651007","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-651007","provider":"Iright","version":"1.0","type":"link"}