{"product_id":"biolegend-651203","title":"Biolegend, 651203, PE anti-ATM Phospho (Ser1981) Antibody, 25μg","description":"\u003cp\u003eAtaxia telangiectasia mutated kinase (ATM) is a serine\/threonine kinase that regulates cell cycle checkpoints and DNA repair. ATM is held inactive in unirradiated cells as a dimer or higher-order multimer. Cellular irradiation induces rapid intermolecular autophosphorylation of serine 1981 that causes dimer dissociation and initiates cellular ATM kinase activity. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KLH conjugated synthetic peptide SLAFEEGSpQSTTISS\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Patent No. USA: 7,108,992\u003cbr\u003e\nProduct Citations: Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed Ryl T et al. 2017. Cell systems. 5(3):237-250 . PubMed Welch G, et al. 2022. Sci Adv. 8:eabo4662. PubMed Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed\u003cbr\u003e\nRRID: AB_2562654 (BioLegend Cat. No. 651203) AB_2562655 (BioLegend Cat. No. 651204)\u003cbr\u003e\nStructure: 3056 amino acids, 350 kD.\u003cbr\u003e\nDistribution: Primarily nuclear, also found in endocytic vesicles.\u003cbr\u003e\nFunction: Serine\/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor.\u003cbr\u003e\nBiology Area: Cell Biology, Chromatin Remodeling\/Epigenetics, Transcription Factors, Ubiquitin\/Protein Degradation\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: 1. Lee JH and Paull TT 2007. Oncogene 26:7741. 2. Bakkenist CJ and Kastan MB 2003. Nature 421:499. 3. McConville CM, et al. 1996. Am. J. Hum. Genet. 59:320. 4. Tang X, et al. 2008. Mol. Cell Biol. 28:2559.\u003cbr\u003e\nGene ID: 472\u003cbr\u003e\nUniProt: View information about ATM on UniProt.org\u003cbr\u003e\nClone: 10H11.E12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ataxia telangiectasia mutated (ATM) kinase, serine-protein kinase, AT mutated\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860783845545,"sku":"651203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-651203","provider":"Iright","version":"1.0","type":"link"}