{"product_id":"biolegend-652406","title":"Biolegend, 652406, APC anti-mouse Ki-67 Antibody, 100μg","description":"\u003cp\u003eThe nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed\u003cbr\u003e\nProduct Citations: Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed Bongiorno EK, et al. 2017. J Immunol. 198:4513. PubMed Daum P, et al. 2023. Front Immunol. 13:991347. PubMed Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Efraim Y, et al. 2022. Cell Rep. 40:111307. PubMed Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Robinson BS, et al. 2020. Am J Pathol. 190:1657. PubMed Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed Li X, et al. 2022. Nat Commun. 13:2794. PubMed Hsu J, et al. 2020. Proceedings of the National Academy of Sciences. 117(38):23626-23635. PubMed Wang W, et al. 2020. Cell Rep. 107936:32. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed Supper E, et al. 2021. Nat Commun. 12:2482. PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed La T, et al. 2018. Cancer Res. 78:6666. PubMed Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed Emmerson E, et al. 2018. EMBO Mol Med. 10.15252\/emmm.201708051. PubMed Bainor AJ et al. 2018. Cell reports. 25(10):2797-2807 . PubMed Wesely J, et al. 2017. Stem Cells Int. 2017:5762301. PubMed Hewitt KJ et al. 2017. Developmental cell. 42(3):213-225 . PubMed Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed Li B, et al. 2019. Oncol Rep. 41:608. PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed Hamaidi I, et al. 2020. Cell Metabolism. 32(3):420-436.e12. PubMed Li J, et al. 2021. Transl Oncol. 14:101116. PubMed Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed Järås M, et al. 2014. J Exp Med. 211:605. PubMed Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed Serr I, et al. 2016. Nat Commun. 7:10991. PubMed Jatho A, et al. 2022. Cells. 11:. PubMed Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed Chen S, et al. 2018. Nat Commun. 9:5298. PubMed Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed Yang F, et al. 2021. Nat Commun. 12:3424. PubMed\u003cbr\u003e\nRRID: AB_2561929 (BioLegend Cat. No. 652405) AB_2561930 (BioLegend Cat. No. 652406)\u003cbr\u003e\nStructure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats\u003cbr\u003e\nDistribution: Nucleus and chromosome\u003cbr\u003e\nFunction: Required for cell cycle progression and proliferation\u003cbr\u003e\nInteraction: Interacts with KIF15; binds to MKI67IP through FHA domain\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143. 2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. 3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. 4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. 5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. 6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.\u003cbr\u003e\nGene ID: 17345\u003cbr\u003e\nUniProt: View information about Ki-67 on UniProt.org\u003cbr\u003e\nClone: 16A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: KIA, proliferation-related Ki-67 antigen\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864352608425,"sku":"652406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-652406","provider":"Iright","version":"1.0","type":"link"}