{"product_id":"biolegend-652409","title":"Biolegend, 652409, FITC anti-mouse Ki-67 Antibody, 25μg","description":"\u003cp\u003eThe nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed\u003cbr\u003e\nProduct Citations: Hu XF, et al. 2021. Theranostics. 3796:11. PubMed Li X, et al. 2023. J Clin Invest. 133:. PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Cautivo KM, et al. 2022. Immunity. 55:254. PubMed Ohkubo R, et al. 2022. Cell Rep. 41:111803. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Bhattacharjee A, et al. 2021. Immunity. 54(8):1745-1757.e7. PubMed Iwasaki Y, et al. 2016. PLoS One. 11: 0158282. PubMed Liu X, et al. 2021. eLife. 0.416666666666667. PubMed Li K, et al. 2022. J Immunother Cancer. 10:. PubMed Li H, et al. 2021. Nat Commun. 12:2773. PubMed Niu S, et al. 2016. J Immunol. 197: 3293 - 3301. PubMed Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed Hu Q, et al. 2022. Diabetes. 71:394. PubMed Yang Z, et al. 2020. Sci Rep. 17757:10. PubMed Zhang T, et al. 2022. Oncogene. . PubMed Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed Webb ER, et al. 2022. iScience. 25:104995. PubMed Han X, et al. 2020. Mol Cells. 43:793. PubMed Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Li K, et al. 2020. Nat Commun. 4.844444444. PubMed Delmas A, et al. 2016. PLoS One. 11: 0163305. PubMed Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed Zhang M, et al. 2022. iScience. 25:104490. PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed\u003cbr\u003e\nRRID: AB_2562140 (BioLegend Cat. No. 652409) AB_2562141 (BioLegend Cat. No. 652410)\u003cbr\u003e\nStructure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats\u003cbr\u003e\nDistribution: Nucleus and chromosome\u003cbr\u003e\nFunction: Required for cell cycle progression and proliferation\u003cbr\u003e\nInteraction: Interacts with KIF15; binds to MKI67IP through FHA domain\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143. 2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. 3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. 4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. 5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. 6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.\u003cbr\u003e\nGene ID: 17345\u003cbr\u003e\nUniProt: View information about Ki-67 on UniProt.org\u003cbr\u003e\nClone: 16A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: KIA, proliferation-related Ki-67 antigen\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864352280745,"sku":"652409","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-652409","provider":"Iright","version":"1.0","type":"link"}