{"product_id":"biolegend-653006","title":"Biolegend, 653006, APC anti-NF-κB p65 Antibody, 100tests","description":"\u003cp\u003eNF-κB p65 is a member of REL-like domain containing protein family, which forms a NF-κB complex with the other family members: NF-κB1 (p105\/p50) or NF-κB2 (p100\/p52). The NF-κB complex is inactivated and held in the cytoplasm by the NF-κB inhibitor IκB. In response to activation stimuli, IκB kinases (IKKs) phosphorylates IκB, resulting in degradation of IκB and liberation of NF-κB complex. The activated NF-κB complex translocates to the nucleus and binds to κB sites in the DNA of their target genes.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human NF-κB p65 recombinant protein (451-551 aa)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nRRID: AB_2563665 (BioLegend Cat. No. 653005) AB_2563666 (BioLegend Cat. No. 653006)\u003cbr\u003e\nStructure: 65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain.\u003cbr\u003e\nDistribution: The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor.\u003cbr\u003e\nFunction: NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses.\u003cbr\u003e\nInteraction: Interacts with NF-κB1 (p105\/p50) or NF-κB2 (p100\/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex.\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184. 2. Saccani S, et al. 2004. J. Exp. Med. 200:107. 3. Nolan GP, et al. 1991. Cell 64:961. 4. Chen LF, et al. 2001. Science 293:1653. 5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593. 6. Chapman NR, et al. 2002. Biochem J. 366:459.\u003cbr\u003e\nGene ID: 4790\u003cbr\u003e\nUniProt: View information about NF-kappaB p65 on UniProt.org\u003cbr\u003e\nClone: 14G10A21\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Nuclear factor NF-kappa-B p65 subunit (NF-κB p65), Rel-A, transcription factor p65, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (NFKB3)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864350118057,"sku":"653006","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-653006","provider":"Iright","version":"1.0","type":"link"}