{"product_id":"biolegend-653305","title":"Biolegend, 653305, Alexa Fluor® 647 anti-mouse Ikaros Antibody, 25μg","description":"\u003cp\u003eIkaros family members, including Ikaros and Helios, play a role in the development of lymphocytes and B and T cells. They are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and IL-2 mediated signaling events.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: N-terminal half of mouse Ikaros fused to GST.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported references (for the relevant formats) include: immunofluorescence1 and immunoprecipitation2.Does not cross-react with human.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).View more applications data for a different format of this clone in our Scientific Poster Library.This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Cobb SB, et al. 2000. Genes \u0026amp; Dev. 14:2146. (IF) Hahm K. et al. 1998. Genes Dev. 12:782. (IP) Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed\u003cbr\u003e\nProduct Citations: M H, et al. 2016. Cell Transplant. 25: 811-27. PubMed\u003cbr\u003e\nRRID: AB_2563162 (BioLegend Cat. No. 653305) AB_2563163 (BioLegend Cat. No. 653306)\u003cbr\u003e\nStructure: Belongs to the Ikaros C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers. 519 amino acids with predicted MW of 58 kD.\u003cbr\u003e\nDistribution: Nucleus. Abundantly expressed in thymus, spleen and peripheral blood leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.\u003cbr\u003e\nFunction: Transcription regulator of hematopoietic cell differentiation. Plays a role in the development of lymphocytes, B and T cells.\u003cbr\u003e\nInteraction: Heterodimer with other IKAROS family members.\u003cbr\u003e\nCell Targets: Component of the chromatin-remodeling NuRD repressor complex which includes at least HDAC1, HDAC2, RBBP4, RBBP7, IKZF1, MTA2, MBD2, MBD3, MTA1L1, CHD3, and CHD4. Interacts directly with the CHD4 component of the NuRD complex.\u003cbr\u003e\nBiology Area: Cell Biology, Chromatin Remodeling\/Epigenetics, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. John LB and Ward AC, 2011. Mol. Immunol. 48:1272. 2. Merkenschlager M, 2010. FEBS Lett. 584:4910. 3. Thomas RM, et al. 2010. J. Biol. Chem. 285:2545. 4. Papathanasiou P, et al. 2009. Stem Cell. 27:3082. 5. Molnar A, et al. 1996. J. Immunol. 156:585.\u003cbr\u003e\nGene ID: 22778\u003cbr\u003e\nUniProt: View information about Ikaros on UniProt.org\u003cbr\u003e\nClone: 2A9\/Ikaros\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IKAROS family zinc finger 1, DNA-binding protein Ikaros\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866540101801,"sku":"653305","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-653305","provider":"Iright","version":"1.0","type":"link"}