{"product_id":"biolegend-653710","title":"Biolegend, 653710, Alexa Fluor® 647 anti-Oct4 (Oct3) Antibody, 100μg","description":"\u003cp\u003eOct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human Oct4 recombinant protein (1-141aa).\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICC - Quality testedICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg\/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Dong T, et al. 2021. Stem Cell Research. 51:102196. PubMed Ervin EH, et al. 2019. Stem Cell Res Ther. 10:43. PubMed\u003cbr\u003e\nRRID: AB_2566689 (BioLegend Cat. No. 653710)\u003cbr\u003e\nStructure: Belongs to the POU transcription factor family, predicted molecular weight of 39 kD\u003cbr\u003e\nDistribution: Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells\u003cbr\u003e\nFunction: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.\u003cbr\u003e\nInteraction: SOX2, SOX8, SOX10, androgen receptor, and ETS2\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Niwa H, et al. 2000. Nature Genet. 24:372. 2. Wernig M, et al. 2007. Nature 448:318. 3. Pesce M, et al. 2001. Stem Cells 19:271.\u003cbr\u003e\nGene ID: 5460\u003cbr\u003e\nUniProt: View information about Oct4 on UniProt.org\u003cbr\u003e\nClone: 3A2A20\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864371318953,"sku":"653710","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-653710","provider":"Iright","version":"1.0","type":"link"}