{"product_id":"biolegend-655902","title":"Biolegend, 655902, Purified anti-Wntless (GPR177) Antibody, 100μg","description":"\u003cp\u003eWnt signaling plays important roles in embryonic development and cancer formation. Wntless (GPR177) is a putative G-protein coupled receptor that regulates Wnt's trafficking and secretion. It can regulate the expression, subcellular location, binding, and organelle-specific association of Wnt proteins. In mammals, Wntless is mainly expressed in submucosa, muscle, ganglia, and nerve cells of the large intestines. Overexpression of Wntless may activate the NF-κB signaling pathway in cancer cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Wntless protein\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The clone recognizes a ~65 kD protein of unidentified origin. Additional reported applications (for relevant formats) include: immunoprecipitation (IP)1 and immunocytochemistry (ICC)1\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Coombs GS, et al. 2010. J. Cell Sci. 123:3357. (ICC, IP)\u003cbr\u003e\nProduct Citations: Wesslowski J, et al. 2020. J Biol Chem. 295:8759. PubMed Glaeser K, et al. 2018. EMBO J. 37:. PubMed\u003cbr\u003e\nRRID: AB_2562228 (BioLegend Cat. No. 655902)\u003cbr\u003e\nStructure: Three isoforms with 450 to 543 amino acids, predicted molecular weight of 45-65 kD; structure similar to the member of G-protein coupled receptor (GPCR) superfamily, with conserved YXXΦ endocytotic motif\u003cbr\u003e\nDistribution: Cytoplasmic organelles\u003cbr\u003e\nFunction: Regulates Wnt protein sorting and secretion; it plays a key role in the regulation of expression, subcellular localization, binding and organelle-specific association of Wnt proteins\u003cbr\u003e\nInteraction: Interacts with WNT1, WNT3, WNT5A, and Vps35\u003cbr\u003e\nLigand\/Receptor: Wnt\u003cbr\u003e\nBiology Area: Angiogenesis, Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, DNA Repair\/Replication, Immunology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Hardin J and King RS, et al. 2008. Curr. Opin. Genet. Dev. 18:362. 2. Wang LT, et al. 2012. Kaohsiung J. Med. Sci. 28:355. 3. Cornett B, et al. 2013. Dev. Biol. 2013. 379:38. 4. Das S, et al. 2012. Front. Biol. (Beijing). 7:587. 5. Stefater JA 3rd, et al. 2013. Blood 121:2574.\u003cbr\u003e\nGene ID: 79971\u003cbr\u003e\nUniProt: View information about Wntless on UniProt.org\u003cbr\u003e\nClone: YJ5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: G Protein-Coupled Receptor 177 (GPR177), WLS, Protein Wntless Homolog, Putative NF-Kappa-B-Activating Protein 373, C1orf139, Evi\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864106029225,"sku":"655902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-655902","provider":"Iright","version":"1.0","type":"link"}