{"product_id":"biolegend-656008","title":"Biolegend, 656008, Alexa Fluor® 647 anti-IRF7 Antibody, 100tests","description":"\u003cp\u003eIRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial IRF7 recombinant protein (127-503 a.a.)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nProduct Citations: Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed\u003cbr\u003e\nRRID: AB_2563530 (BioLegend Cat. No. 656007) AB_2563531 (BioLegend Cat. No. 656008)\u003cbr\u003e\nStructure: 503 amino acids, predicted molecular weight of 54 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding\u003cbr\u003e\nDistribution: Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus\u003cbr\u003e\nFunction: Transcription factor that transactivates proinflamatory cytokines and type I interferon genes in response to virus infection\u003cbr\u003e\nInteraction: Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Owens BM, et al. 2012. PLoS One. 7:e41050. 2. Yu Y, et al. 2010. Immunity. 33:863. 3. Liang Q, et al. 2011. J. Immunol. 186:1001. 4. Buss C, et al. 2010. J. Immunol. 184:3072. 5. Joo CH, et al. 2007. J. Virol. 81:8282. 6. Balachandran S, et al. 2007. J. Immunol. 178:2429.\u003cbr\u003e\nGene ID: 3665\u003cbr\u003e\nUniProt: View information about IRF7 on UniProt.org\u003cbr\u003e\nClone: 12G9A36\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon regulatory factor-7 (IRF-7)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864329081001,"sku":"656008","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-656008","provider":"Iright","version":"1.0","type":"link"}