{"product_id":"biolegend-658704","title":"Biolegend, 658704, Alexa Fluor® 488 anti-Bcl-2 Antibody, 100tests","description":"\u003cp\u003eBcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC) Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Tsuzuki H, et al. 2022. Oncotarget. 13:1359. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed\u003cbr\u003e\nRRID: AB_2563151 (BioLegend Cat. No. 658703) AB_2563152 (BioLegend Cat. No. 658704)\u003cbr\u003e\nStructure: Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.\u003cbr\u003e\nDistribution: Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.\u003cbr\u003e\nFunction: Regulates apoptosis through controlling mitochondrial fusion and fission.\u003cbr\u003e\nInteraction: BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Chromatin Remodeling\/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin\/Protein Degradation\u003cbr\u003e\nAntigen References: 1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243. 2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7. 3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414. 4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571. 5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.\u003cbr\u003e\nGene ID: 596\u003cbr\u003e\nUniProt: View information about Bcl-2 on UniProt.org\u003cbr\u003e\nClone: 100\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B-Cell CLL\/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50\u003cbr\u003e\nIsotype: Mouse IgG1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860770246825,"sku":"658704","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-658704","provider":"Iright","version":"1.0","type":"link"}