{"product_id":"biolegend-663905","title":"Biolegend, 663905, Purified anti-, 100μg","description":"\u003cp\u003eRNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: E. coli\u003cbr\u003e\nReported Reactivity: Other species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified recombinant E. coli RNA polymerase β subunit.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in phosphate-buffered solution, pH 7.2.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.\u003cbr\u003e\nApplication: WB - Quality tested ELISA, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)\u003cbr\u003e\nProduct Citations: Cui Y, et al. 2022. Appl Environ Microbiol. 88:e0130522. PubMed Wong AI, et al. 2023. Elife. 12:. PubMed Dupuy P, et al. 2023. Elife. 12:. PubMed Tang MX, et al. 2022. ISME J. 16:1765. PubMed Morreale FE, et al. 2022. Cell. 185:2338. PubMed Chihara K, et al. 2022. RNA. :. PubMed d'Andrea FB, et al. 2022. Sci Adv. 8:eabn7943. PubMed Dupuy P, et al. 2020. Proc Natl Acad Sci U S A. 117:19517. PubMed Wipperman MF et al. 2018. Molecular cell. 72(1):152-161 . PubMed Olivencia B,et al. 2017. Sci Rep.. 10.1038\/s41598-017-14410-z. PubMed Liang X, et al. 2021. PLoS Pathog. 17:e1010116. PubMed Hör J, et al. 2020. Nucleic Acids Res. 48:9301. PubMed Dupuy P, et al. 2022. Nat Commun. 13:4493. PubMed Liang X, et al. 2021. Appl Environ Microbiol. 87:e0043521. PubMed\u003cbr\u003e\nRRID: AB_2566583 (BioLegend Cat. No. 663905)\u003cbr\u003e\nStructure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.\u003cbr\u003e\nDistribution: Bacteria.\u003cbr\u003e\nFunction: Transcriptional regulator in bacteria.\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.\u003cbr\u003e\nGene ID: 948488\u003cbr\u003e\nUniProt: View information about RNA Polymerase beta on UniProt.org\u003cbr\u003e\nClone: 8RB13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862496497833,"sku":"663905","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-663905","provider":"Iright","version":"1.0","type":"link"}