{"product_id":"biolegend-663907","title":"Biolegend, 663907, Direct-Blot™ HRP anti-, 100microl","description":"\u003cp\u003eRNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: E. coli\u003cbr\u003e\nReported Reactivity: Other species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified recombinant E. coli RNA polymerase β subunit.\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nApplication Notes: Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)\u003cbr\u003e\nRRID: AB_2629625 (BioLegend Cat. No. 663907)\u003cbr\u003e\nStructure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.\u003cbr\u003e\nDistribution: Bacteria.\u003cbr\u003e\nFunction: Transcriptional regulator in bacteria.\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.\u003cbr\u003e\nGene ID: 948488\u003cbr\u003e\nUniProt: View information about RNA Polymerase beta on UniProt.org\u003cbr\u003e\nClone: 8RB13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862496530601,"sku":"663907","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-663907","provider":"Iright","version":"1.0","type":"link"}