{"product_id":"biolegend-664803","title":"Biolegend, 664803, Direct-Blot™ HRP anti-β-actin Antibody, 25microl","description":"\u003cp\u003eβ-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).\u003cbr\u003e\n25microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, Zebrafish, Drosophila\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human ACTB peptide (1-13 a.a.).\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml (1:10,000 - 1:100,000 dilution). It is recommended that the reagent be titrated for optimal performance for each application. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nApplication Notes: Reactivity to Drosophila was only verified with the purified format.\u003cbr\u003e\nProduct Citations: Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed Li Y, et al. 2020. Stem Cell Res Ther. 0.815972222. PubMed Sripathi SR, et al. 2021. Mol Cell Proteomics. 20:100131. PubMed Wijeratne TU, et al. 2022. J Biol Chem. :102319. PubMed\u003cbr\u003e\nRRID: AB_2810693 (BioLegend Cat. No. 664803) AB_2728496 (BioLegend Cat. No. 664804)\u003cbr\u003e\nStructure: 375 amino acids with a predicted molecular weight of 42 kD.\u003cbr\u003e\nDistribution: Cytoplasm\u003cbr\u003e\nFunction: Actins are highly conserved proteins that are involved in cell motility.\u003cbr\u003e\nInteraction: Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Microfilaments\u003cbr\u003e\nAntigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.\u003cbr\u003e\nGene ID: 60\u003cbr\u003e\nUniProt: View information about beta-actin on UniProt.org\u003cbr\u003e\nClone: W16197A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ACTB, Actin, cytoplasmic 1, Beta-actin\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866306859177,"sku":"664803","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-664803","provider":"Iright","version":"1.0","type":"link"}