{"product_id":"biolegend-671501","title":"Biolegend, 671501, Purified anti-SEC13 Antibody, 25μg","description":"\u003cp\u003eSEC13 functions as a component of the nuclear pore complex (NPC) involved in nucleo-cytoplasmic trafficking. At the ER, SEC13 is involved in the biogenesis of COPII-coated vesicles, which are required for protein export from the ER to either the Golgi complex or the ER-Golgi intermediate compartment (ERGIC). It also functions as a component of the GATOR2 complex, which acts as an amino acid-sensing branch of the TOR1 signaling pathway.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial-length human SEC13 recombinant protein at 123-322 a.a.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone failed IP testing when the target was pulled down and probed via WB.In addition, a nonspecific band was detected in the initial Western Blot screen; however, that band was titrated out at ~0.1 µg\/mL. Nevertheless, the presence of the band indicates that the clone is not suitable for ICC testing.\u003cbr\u003e\nRRID: AB_2814534 (BioLegend Cat. No. 671501) AB_2814535 (BioLegend Cat. No. 671502)\u003cbr\u003e\nStructure: SEC13 is a 322 amino-acid protein with a predicted molecular weight of 39 kD.\u003cbr\u003e\nDistribution: Localized to the nucleoplasm and vesicles; ubiquitously expressed.\u003cbr\u003e\nFunction: mTOR signaling; vesicle biogenesis\u003cbr\u003e\nBiology Area: Protein Trafficking and Clearance\u003cbr\u003e\nAntigen References: Swaroop A, et al. 1994. Hum. Mol. Genet. 3(8):1281-6. Wolfson RL, et al. 2017. Nature 543(7645): 438-442. Cho HJ, et al. 2014. EMBO J 33(20):2314-31. Chantranupong L, et al. 2014. Cell Rep. 9(1):1-8. Bar-Peled L, et al. 2013. Science. 340(6136):1100-6.\u003cbr\u003e\nGene ID: 6396\u003cbr\u003e\nUniProt: View information about SEC13 on UniProt.org\u003cbr\u003e\nClone: W18034A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SEC13-like protein 1, SEC13-related protein; nuclear pore and COPII coat complex component\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866329927849,"sku":"671501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-671501","provider":"Iright","version":"1.0","type":"link"}