{"product_id":"biolegend-671953","title":"Biolegend, 671953, Ultra-LEAF™ Purified anti-mouse IFNgR2 Antibody, 100μg","description":"\u003cp\u003eIFN-γ initiates and regulates a variety of immune responses. IFNγ receptor is a heterodimer of high affinity IFNγ-binding chain (IFNγR1) and a second accessory protein required for signal transduction known as IFNγR2 (IFN-γ R β chain). IFNγR1 and IFNγR2 belongs to a member of the type II cytokine receptor family. IFNγR1 is constitutively expressed on the surface of T cells, B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells at the moderate level. IFNγR2 is has low levels of cell surface and high levels of cytoplasmic expression. Though the recycling of IFNγR2 between cell surface and cytoplasm is IFN-γ-independent, it depends on a dipeptide motif on its intracellular domain and its expression is tightly regulated by external stimuli. IFN-γ can decrease mRNA expression of IFNγR2, and modulates T cell responsiveness to IFN-γ. Binding of IFN-γ induces receptor-ligand dimerization, tyrosine phosphorylation of IFNγR1 and internalization. IFNγR2 is resposible for signal transduction, involving Jak1\/ Jak2 protein kinases and STAT1 activation. T cells isolated from IFNγR2 deficient mice, or from transgenic mouse (over-expressing IFNγR2) demonstrate impaired capacity to differentiate into the Th1 cells.  Medndelian susceptibility to mycobacterial disease (MSDS) syndrome in human  has been linked to a autosomal recessive and autosomal dominant deficiency of IFNγR2.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant Mouse IFNgR2\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/μg of the protein (less than 0.001 ng\/μg of the protein) as determined by the LAL test\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: Block - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 8 µg\/mL mouse IFNgR2 in the presence of 0.2 μg\/ml mouse IFN-γ (Cat. No. 575304) to immobilized 3 µg\/mL recombinant mouse IFNgR1. ND50 range: 1.2 - 7 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_3699086 (BioLegend Cat. No. 671953) AB_3699086 (BioLegend Cat. No. 671954)\u003cbr\u003e\nStructure: Type II cytokine receptor family\u003cbr\u003e\nDistribution: T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells; plasma membrane, cytoplasm, ER\u003cbr\u003e\nFunction: IFNγR2 forms a heterodimer with IFNγR1.\u003cbr\u003e\nInteraction: Intracellular domain of IFNγR2 interacts with JAK2.\u003cbr\u003e\nLigand\/Receptor: IFN-γ\u003cbr\u003e\nCell Type: Astrocytes, B cells, Endothelial cells, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Microglia, Monocytes, Neutrophils, NK cells, NKT cells, T cells, Th1\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology, Innate Immunity, Neuroinflammation\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors, Innate Immune Signaling\u003cbr\u003e\nAntigen References: Hemmi S, et al. 1994. Cell. 76:803-10. Soh J, et al. 1994. Cell. 76:793-802. Rigamonti L, et al. 2000. J Immunol. 164:201-7. Foulds KE, et al. 2008. J Immunol. 180:842-9. Rosenzweig SD, et al. 2004. J Immunol. 173:3991-9. Haring JS, et al. 2005. J Immunol. 174:6791-802. Kotenko SV, et al. 1995. J Biol Chem. 270:20915-21. Sakatsume M, et al. 1995. J Biol Chem. 270:17528-34. Schroder K, et al. 2004. J Leuko Biol. 75:163-89. Vogt G, et al. 2005. Nat Genet. 37:692-700. Dorman SE, et al. 1998. J Clin Invest. 101:2364-9. Tau G, et al. 1999. Allergy. 54:1233-51. Tau GZ, et al. 2000. J Exp Med. 192:977-86.\u003cbr\u003e\nGene ID: 15980\u003cbr\u003e\nUniProt: View information about IFNgR2 on UniProt.org\u003cbr\u003e\nClone: W20010A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon gamma receptor 2, IFN-gamma receptor 2, IFN-gamma-R2, Interferon gamma receptor accessory factor 1, AF-1, Interferon gamma receptor beta chain, IFN-gamma-R-beta, IFN-γR2, Interferon gamma transducer 1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866417615017,"sku":"671953","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-671953","provider":"Iright","version":"1.0","type":"link"}