{"product_id":"biolegend-674001","title":"Biolegend, 674001, Purified anti-Nanog Antibody, 25μg","description":"\u003cp\u003eNanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length human recombinant protein expressed in bacteria\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted at 4°C\u003cbr\u003e\nApplication: WB - Quality tested ICFC, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is ≤ 0.175 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nRRID: AB_2564570 (BioLegend Cat. No. 674001) AB_2564571 (BioLegend Cat. No. 674002)\u003cbr\u003e\nStructure: 305 amino acids with a predicted molecular weight of approximately 35 kD.\u003cbr\u003e\nDistribution: Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.\u003cbr\u003e\nFunction: Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.\u003cbr\u003e\nInteraction: Interacts with SMAD1, SALL4, and ZNF281\/ZFP281.\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Hwang CC, et al. 2014. PLoS One 9:e105676. 2. Torres-Padilla ME, et al. 2014. Development 141:2173. 3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741. 4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207. 5. Kim J, et al. 2008. Cell 132:1049. 6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081. 7. Yu J, et al. 2007. Science 318:1917.\u003cbr\u003e\nGene ID: 79923\u003cbr\u003e\nUniProt: View information about Nanog on UniProt.org\u003cbr\u003e\nClone: 23D2-3C6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860872057001,"sku":"674001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-674001","provider":"Iright","version":"1.0","type":"link"}