{"product_id":"biolegend-675506","title":"Biolegend, 675506, Direct-Blot™ HRP anti-ERK1\/2 Phospho (Thr202\/Tyr204) Antibody, 25microl","description":"\u003cp\u003eERK1\/2 are members of mitogen-activated kinases (MAPKs) of serine\/threonine protein kinases. ERK1\/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1\/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.\u003cbr\u003e\n25microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nProduct Citations: Chyuan IT, et al. 2024. J Biomed Sci. 33:31. PubMed Beà A, et al. 2022. FEBS J. 289:2540. PubMed\u003cbr\u003e\nRRID: AB_2801130 (BioLegend Cat. No. 675506) AB_2566700 (BioLegend Cat. No. 675505)\u003cbr\u003e\nStructure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.\u003cbr\u003e\nDistribution: Cytoplasm. Nucleus.\u003cbr\u003e\nFunction: ERK1\/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1\/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.\u003cbr\u003e\nInteraction: ERK1\/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology\u003cbr\u003e\nMolecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.\u003cbr\u003e\nGene ID: 5594\u003cbr\u003e\nUniProt: View information about ERK1\/2 on UniProt.org\u003cbr\u003e\nClone: 4B11B69\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: P44\/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864373088425,"sku":"675506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-675506","provider":"Iright","version":"1.0","type":"link"}