{"product_id":"biolegend-677051","title":"Biolegend, 677051, Purified anti-HIF2A Antibody, 25μg","description":"\u003cp\u003eHIF2A is a transcription factor that mediates the cellular adaptive response to hypoxic conditions, orchestrating the transcription of a wide array of target genes to enable cellular survival and homeostatic maintenance in low-oxygen environments. At an organismal level, HIF2A is also involved in essential physiological processes such as erythropoiesis and angiogenesis. Under normoxic conditions, HIF2A undergoes prolyl hydroxylation which leads to its degradation by the proteasome. Oxygen starvation inhibits prolyl hydroxylation and prevents HIF2A degradation, leading to stabilization of the protein and translocation to the nucleus to alter gene expression. Dysregulation of HIF2A has been implicated in various pathological states including cancer, where it promotes tumor growth by enhancing the ability of tumor cells to survive hypoxic conditions within the tumor microenvironment.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of the C-terminus of human HIF2A\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody is not recommended for use in immunocytochemistry (ICC), intracellular flow cytometry (ICFC), or immunohistochemistry in formalin-fixed, paraffin-embedded tissues (IHC-P).\u003cbr\u003e\nRRID: AB_3683496 (BioLegend Cat. No. 677051) AB_3683496 (BioLegend Cat. No. 677052)\u003cbr\u003e\nStructure: HIF2A is an 870 amino acid protein with a predicted molecular weight of 96.5 kD.\u003cbr\u003e\nDistribution: Cytoplasmic, translocates to nucleus upon stabilization\u003cbr\u003e\nFunction: Transcription factor\u003cbr\u003e\nInteraction: Dimerizes with HIF1B upon hypoxic induction. Under normoxic conditions, undergoes prolyl hydroxylation by Von Hippel-Lindau (VHL)\u003cbr\u003e\nCell Type: Endothelial cells\u003cbr\u003e\nBiology Area: Signal Transduction, Transcription Factors\u003cbr\u003e\nAntigen References: Tian H, et al. 1997. Genes Dev. 11:72-82. Wiesener, et al. 1998. Blood. 92:2260-8. Giatromanolaki A and Harris AL 2001. Anticancer Res. 6B:4317-24. MacInnis MJ and Rupert JL. 2011. High Alt Med Biol. 12:133-9.\u003cbr\u003e\nGene ID: 2034\u003cbr\u003e\nUniProt: View information about HIF2A on UniProt.org\u003cbr\u003e\nClone: W22144B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Q99814, Hypoxia induficble factor 2-alpha (HIF2A, HIF2α), Endothelial PAS domain-containing protein 1 (EPAS1), class E basic helix-loop-helix protein 73 (bHLHe73), Member of PAS protein 2 (MOP2), PAS domain-containing protein 2 (PASD2), BHLHE73, HIF2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866434064553,"sku":"677051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-677051","provider":"Iright","version":"1.0","type":"link"}