{"product_id":"biolegend-685502","title":"Biolegend, 685502, Purified anti-HDAC8 Antibody, 100μg","description":"\u003cp\u003eHistone deacetylases (HDACs) are a family of enzymes responsible for the removal of acetyl groups from lysine residues on the core histones and other non-histone proteins. HDAC8 is a type I histone deacetylase and was initially found to deacetylate a variety of acetylated histone variants in vitro . HDAC8 is a kinectically and structurally well-characterized deacetylase that was reported to catalyze several nuclear proteins, which include ERR-α, hEST1B, and SMC3. Patients with HDAC8 mutations show Cornelia de Lange Syndrome (CdLS) and Wilson-turner Syndrome (WTS) phenotypes. Elevated expression of HDAC8 has been observed in various tumor types, such as acute lymphoblastic leukemia (AML), neuroblastomas, and glimoas. HDAC8 is implicated as a therapeutic target in cancer and infectious diseases.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length human HDAC8 recombinant protein expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested KO\/KD-WB, ICC, ChIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/ml is recommended. For ChIP applications, the suggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Aru B, et al. 2020. ACS Omega. 5:25854. PubMed\u003cbr\u003e\nRRID: AB_2572204 (BioLegend Cat. No. 685502)\u003cbr\u003e\nStructure: 377 amino acids with a predicted molecular weight of 41.8 kD. It belongs to the type I Histone deacetylase subfamily.\u003cbr\u003e\nDistribution: Nucleus and cytoplasm.\u003cbr\u003e\nFunction: HDAC8 is a deacetylase and regulates gene expression through catalyzing the removal of acetyl groups on lysine residues in the core histones and transcriptional factors.\u003cbr\u003e\nInteraction: Interacts with PEPB2-MYH11, CBFA2T3, SMG5, and alpha-SMA.\u003cbr\u003e\nBiology Area: Cell Biology, Chromatin Remodeling\/Epigenetics, Neuroscience, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Qi J, et al. 2015. Cell Stem Cell. 17:597. 2. Tian Y, et al. 2015. Cancer Res. 75:4803. 3. Feng L, et al. 2015. J. Hum. Genet. 60:165. 4. Ha SD, et al. 2014. J. Immunol. 193:1333. 5. Higuchi T, et al. 2013. Blood 121:3640. 6. Deardorff MA, et al. 2012. Nature 489:313.\u003cbr\u003e\nGene ID: 55869\u003cbr\u003e\nUniProt: View information about HDAC8 on UniProt.org\u003cbr\u003e\nClone: W15090A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Histone deacetylase-like 1 (HDACL1), MRXS6, Wilson-Turner X-linked mental retardation syndrome (WTS), RPD3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866297847977,"sku":"685502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-685502","provider":"Iright","version":"1.0","type":"link"}