{"product_id":"biolegend-686402","title":"Biolegend, 686402, Purified anti-STAT1 Phospho (Ser727) Antibody, 100μg","description":"\u003cp\u003eSTAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human STAT1 peptide phosphorylated at Ser 727.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICFC, ICC, ChIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg\/ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg\/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Morisaki Y, et al. 2023. Front Cell Neurosci. 1308972:17. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Wu Y, et al. 2021. J Clin Invest. 131:. PubMed Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed\u003cbr\u003e\nRRID: AB_2861070 (BioLegend Cat. No. 686401) AB_2616883 (BioLegend Cat. No. 686402)\u003cbr\u003e\nStructure: 750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.\u003cbr\u003e\nDistribution: Translocates to the nucleus when phosphorylated.\u003cbr\u003e\nFunction: Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α\/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.\u003cbr\u003e\nInteraction: Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Nuclear Markers, Phospho-Proteins\u003cbr\u003e\nAntigen References: 1. Durbin JE, et al. 1996. Cell. 84:443. 2. Darnell JE Jr, et al. 1994. Science 264:1415. 3. Chen X, et al. 1998. Cell. 93:827. 4. Ramana CV, et al. 2000. Oncogene. 19:2619.\u003cbr\u003e\nGene ID: 6772\u003cbr\u003e\nUniProt: View information about STAT1 Phospho Ser727 on UniProt.org\u003cbr\u003e\nClone: A15158B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91\/p84\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864390652073,"sku":"686402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-686402","provider":"Iright","version":"1.0","type":"link"}