{"product_id":"biolegend-688202","title":"Biolegend, 688202, Purified anti-Strep-Tag Antibody, 25μg","description":"\u003cp\u003eThe Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic Peptide\u003cbr\u003e\nFormulation: PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.\u003cbr\u003e\nPreparation: The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Wang WA, et al. 2023. J Cell Sci. 136:. PubMed Liu Y, et al. 2021. MBio. 12:e0233521. PubMed Dominguez Andres A, et al. 2020. bioRxiv. . PubMed Xu Z, et al. 2022. Front Immunol. 13:1047922. PubMed Xu Z, et al. 2021. Cell Death Dis. 12:57. PubMed\u003cbr\u003e\nRRID: AB_2629594 (BioLegend Cat. No. 688202)\u003cbr\u003e\nStructure: The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.\u003cbr\u003e\nFunction: Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nAntigen References: 1. Skerra A, et al. 2000. Methods Enzymol. 326:271. 2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Strep-Tag on UniProt.org\u003cbr\u003e\nClone: BL26882\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866451890345,"sku":"688202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-688202","provider":"Iright","version":"1.0","type":"link"}