{"product_id":"biolegend-689202","title":"Biolegend, 689202, HRP Protein A, 500microl","description":"\u003cp\u003eProtein A is a 42 kD surface protein originally found in the cell wall of the bacterium Staphylococcus aureus . It is widely used in biochemical research and industry because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. It binds the heavy chain within the Fc region of most immunoglobulins. HRP Protein A is useful for detecting primary antibodies, e.g. , in ELISA, ELISPOT, IHC, or Western blotting. For IP followed by Western blotting application, HRP protein A will avoid IgG heavy\/light chain issue because it only recognizes the IgG in its native form, and the antibody used to IP the target is usually boiled with the immunoprecipitates and gets denatured.\u003cbr\u003e\n500microl\u003cbr\u003e\nVerified Reactivity: Mouse, Rabbit\u003cbr\u003e\nFormulation: This reagent is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: Recombinant protein A was conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: For Western blot analysis, the reagent should be titrated between 1:2000 - 1:8000 to determine optimal conditions. Affinity of Protein A to Ig Types from Different Species: Species Immunoglobulin Binding to Protein A Mouse IgG1 + IgG2a ++++ IgG2b +++ IgG3 ++ IgM - Rat IgG1 - IgG2a - IgG2b - IgG2c ++ Human Total IgG ++++ IgG1 ++++ IgG2 ++++ IgG3 - IgG4 ++++ IgM - IgA - IgE - Chicken IgY - Goat IgG +\/- Hamster IgG + Rabbit IgG ++++ Sheep IgG +\/-\u003cbr\u003e\nProduct Citations: Bauer A, et al. 2023. Pharmaceutics. :15. PubMed\u003cbr\u003e\nStructure: 42 kD surface protein.\u003cbr\u003e\nFunction: Binds IgG.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nAntigen References: 1. Verwey WF. 1940. J. Exp. Med. 71:635. 2. Graille M, et al. 2000. Proc. Natl. Acad. Sci. USA 97:5399.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Protein A on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein A\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866442879145,"sku":"689202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-689202","provider":"Iright","version":"1.0","type":"link"}