{"product_id":"biolegend-691602","title":"Biolegend, 691602, Purified anti-Artemis Antibody, 100μg","description":"\u003cp\u003eArtemis, also known as DCLRE1C, is a nuclear protein belonging to the metallo-β-lactamase superfamily. Artemis has been found to be required for the assembling of B cell antibody genes and T cell receptor genes from individual V, D, and J segments. During V(D)J recombination, Artemis forms a complex with DNA-dependent protein kinase (DNA-‘PK) and cuts the DNA with hairpin structure. Deficiency in Artemis results in blocking of V(D)J recombination and failure to produce mature B and T cells, which is known as the condition of severe combined immune deficiency (SCID). Artemis is also found to be involved in the termini processing step of double-strand break (DSB) repair. Artemis-deficient cells are more sensitive to DSB induced by ionizing radiation or chemical agents such as bleomycin and neocarzinostatin.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human Artemis recombinant protein (347-692 aa) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody has a similar affinity to BioLegend clone 13A9C90 for human Artemis by western blot, but does not recognize mouse Artemis\u003cbr\u003e\nProduct Citations: Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed\u003cbr\u003e\nRRID: AB_2632653 (BioLegend Cat. No. 691602)\u003cbr\u003e\nStructure: 692 amino acids with a predicted molecular weight of 78.4 kD. Four isoforms produced by alternative splicing.\u003cbr\u003e\nDistribution: Nucleus.\u003cbr\u003e\nFunction: Artemis is a nuclear protein exhibiting single-strand-specific 5'-3' exonuclease activity and endonuclease activity. It plays crucial roles in V(D)J recombination and DNA repair.\u003cbr\u003e\nInteraction: Interacts with ATM, BRCA1, PRKDC and TP53BP1.\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication\u003cbr\u003e\nAntigen References: 1. Chang HH, et al. 2016. Nucleic Acids Res. 44:4991. 2. Volk T, et al. 2015. Hum. Mol. Genet. 24:7361. 3. Waide EH, et al. 2015. J. Immunol. 195:3171. 4. Lee PP, et al. 2013. Clin. Immunol. 149:464. 5. Köcher S, et al. 2012. Nucleic Acids Res. 40:8336. 6. Beucher A, et al. 2009. EMBO J. 28:3413.\u003cbr\u003e\nGene ID: 64421\u003cbr\u003e\nUniProt: View information about Artemis on UniProt.org\u003cbr\u003e\nClone: 15E7C90\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein artemis, DNA cross-link repair 1C protein (DCLRE1C), ASCID, SCIDA, SNM1 homolog C (SNM1C)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864351592617,"sku":"691602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-691602","provider":"Iright","version":"1.0","type":"link"}