{"product_id":"biolegend-692051","title":"Biolegend, 692051, Purified anti-Calponin 1 Antibody, 25μg","description":"\u003cp\u003eCalponin 1, encoded by the CNN1 gene, is an actin filament-associated regulatory protein found primarily in smooth muscle but also in non-muscle cells. Calponin 1 plays a significant role in regulating the contractility and structural integrity of the cytoskeleton by binding to actin and actin-interacting proteins, modulating their interplay. For example, by binding to actin and inhibiting myosin light chain kinase Calponin 1 reduces the phosphorylation of myosin light chains, preventing excessive contraction and ensuring appropriate smooth muscle response to physiological stimuli. Furthermore, Calponin 1 has been implicated in various physiological functions beyond muscle contraction including vascular tone and wound healing, making it a key player in maintaining homeostasis in various tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Crude human uterus extract\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICC, WB, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg\/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with any of the following: - Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 - Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol - 100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix\/permeabilize with Cyto-Fast™ Fix\/Perm Buffer Set (Cat. No. 426803). Fixation\/permeabilization with either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended. This antibody does not cross react with mouse Calponin 1 in Western Blot.\u003cbr\u003e\nRRID: AB_3683499 (BioLegend Cat. No. 692051) AB_3683499 (BioLegend Cat. No. 692052)\u003cbr\u003e\nStructure: Calponin 1 is a 297 amino acid protein with a calculated molecular weight of 33.1 kD.\u003cbr\u003e\nDistribution: Cytoplasmic, cytoskeleton\u003cbr\u003e\nInteraction: Actin, tropomyosin, myosin, calmodulin\u003cbr\u003e\nBiology Area: Cardiovascular Biology, Cell Motility\/Cytoskeleton\/Structure\u003cbr\u003e\nMolecular Family: Microfilaments\u003cbr\u003e\nAntigen References: Wu K and Jin J. 2008. Cell Biochem Biophys. 52:139-48. Liu R and Jin JP. 2016. Gene. 585:143-153. Winder SJ and Walsh M. 1993. Cell Signal. 5:677-86.\u003cbr\u003e\nGene ID: 1264\u003cbr\u003e\nUniProt: View information about Calponin 1 on UniProt.org\u003cbr\u003e\nClone: CALP\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Calponin 1, basic, smooth muscle (CNN1), Epididymis Secretory Protein Li 14, SMCC, Sm-Calp, HEL-S-14, Basic calponin, Calponin H1 smooth muscle\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862961410217,"sku":"692051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-692051","provider":"Iright","version":"1.0","type":"link"}