{"product_id":"biolegend-693504","title":"Biolegend, 693504, PE anti-ZAP-70 Antibody, 100tests","description":"\u003cp\u003eZAP-70 is a 70kD protein expressed near the surface membrane of T-cells and NK cells. It is part of the intracellular signaling mechanism of the T-cell receptor, and plays integral role in T-cell signaling where it interacts with CD3-ζ to phosphorylate transmembrane protein LAT, which facilitates the binding of many signaling proteins. ZAP-70 plays a key role in the activation of T-cells, and their subsequent proliferation, differentiation, and production of cytokines in response to varied stimuli. It is used clinically as a prognostic marker helping differentiate the various types of chronic lymphocytic leukemia (CLL).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human ZAP 70 peptide phosphorylated at Y493. Complete Freund's adjuvant.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: CD3, CD19, and CD56 co-staining indicates that this clone does not bind B-cells and will not bind Syk. Clone A16043B cross-reacts with mouse species.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone, A16043B, recognizes both non-phosphorylated ZAP-70 and phosphorylated forms of ZAP-70.\u003cbr\u003e\nRRID: AB_2632836 (BioLegend Cat. No. 693503) AB_2632837 (BioLegend Cat. No. 693504)\u003cbr\u003e\nStructure: Protein tyrosine kinase, contains two SH2 domains, 70 kD.\u003cbr\u003e\nDistribution: T cells, NK cells, and B-cell chronic lymphocytic leukemia (B-CLL).\u003cbr\u003e\nFunction: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.\u003cbr\u003e\nLigand\/Receptor: Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc.\u003cbr\u003e\nCell Type: T cells, NK cells, Leukemia\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC) 2. Crespo M, et al. 2003. N. Engl. J. Med. 348:1764. (WB, IP) 3. Jamroziak K, et al. 2009. Cancer Epidemiol. Biomarkers Prev. 18:945.\u003cbr\u003e\nGene ID: 753522637\u003cbr\u003e\nUniProt: View information about ZAP-70 on UniProt.org\u003cbr\u003e\nClone: A16043B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Zeta chain of TCR associated protein kinase 70\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864393732265,"sku":"693504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-693504","provider":"Iright","version":"1.0","type":"link"}