{"product_id":"biolegend-693507","title":"Biolegend, 693507, Alexa Fluor® 647 anti-ZAP-70 Antibody, 25tests","description":"\u003cp\u003eZAP-70 is a 70kD protein expressed near the surface membrane of T-cells and NK cells. It is part of the intracellular signaling mechanism of the T-cell receptor, and plays integral role in T-cell signaling where it interacts with CD3-ζ to phosphorylate transmembrane protein LAT, which facilitates the binding of many signaling proteins. ZAP-70 plays a key role in the activation of T-cells, and their subsequent proliferation, differentiation, and production of cytokines in response to varied stimuli. It is used clinically as a prognostic marker helping differentiate the various types of chronic lymphocytic leukemia (CLL).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human ZAP 70 peptide phosphorylated at Y493. Complete Freund’s adjuvant.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: CD3, CD19, and CD56 co-staining indicates that this clone does not bind B-cells and will not bind Syk. Clone A16043B cross-reacts with mouse species. This clone, A16043B, recognizes both non-phosphorylated ZAP-70 and phosphorylated forms of ZAP-70.\u003cbr\u003e\nRRID: AB_2715959 (BioLegend Cat. No. 693507) AB_2715960 (BioLegend Cat. No. 693508)\u003cbr\u003e\nStructure: Protein tyrosine kinase, contains two SH2 domains, 70 kD.\u003cbr\u003e\nDistribution: T cells, NK cells, and B-cell chronic lymphocytic leukemia (B-CLL).\u003cbr\u003e\nFunction: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.\u003cbr\u003e\nLigand\/Receptor: Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc.\u003cbr\u003e\nCell Type: Leukemia, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC) 2. Crespo M, et al. 2003. N. Engl. J. Med. 348:1764. (WB, IP) 3. Jamroziak K, et al. 2009. Cancer Epidemiol. Biomarkers Prev. 18:945.\u003cbr\u003e\nGene ID: 753522637\u003cbr\u003e\nUniProt: View information about ZAP-70 on UniProt.org\u003cbr\u003e\nClone: A16043B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Zeta chain of TCR associated protein kinase 70\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864393863337,"sku":"693507","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-693507","provider":"Iright","version":"1.0","type":"link"}