{"product_id":"biolegend-693802","title":"Biolegend, 693802, Purified anti-ZAP70 Phospho (Tyr292) Antibody, 100μg","description":"\u003cp\u003eZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn.  ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr315 and Tyr319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr292 has a negative regulatory role.  Mutation of Tyr292 to phenylalanine enhanced NFAT induction in response to BCR stimulation, indicating phosphorylation of Tyr292 may promote association of ZAP-70 with a negative regulatory protein. ZAP-70 has been reported to bind Cbl following TCR stimulation and phosphorylation of Tyr292 was required for this interaction, as well as for the negative effect of Cbl on signaling.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human ZAP70 peptide phosphorylated at Tyr292. Complete Freund's adjuvant.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested Direct ELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: During development, reactivity was observed with mouse phospho-SYK.\u003cbr\u003e\nRRID: AB_2686945 (BioLegend Cat. No. 693802)\u003cbr\u003e\nStructure: 619 amino acids with a predicted molecular weight of 70 kD.\u003cbr\u003e\nDistribution: Cytoplasm; Human T Cells, Mouse T cells and B cells.\u003cbr\u003e\nFunction: Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-γ1-dependent and Ras-dependent signaling cascades. Associates with PLC-γ 1.\u003cbr\u003e\nInteraction: NFAM1, Cbl, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, CD247\/CD3Z.\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Phospho-Proteins, Protein Kinases\/Phosphatase, TCRs\u003cbr\u003e\nAntigen References: 1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:9166. 2. Arpaia E, et al. 1994. Cell 76:947. 3. Chan AC, et al. 1994. Science 264:1599. 4. Negishi I, et al. 1995. Nature 376:435. 5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279. 6. Klammt C, et al. 2015. Nat. Immunol. 16:961. 7. Weiss A, et al. 1993. Cell 73:209. 8. Gong Q, et al. 2001. J. Exp. Med. 194:507. 9. Magnan A, et al. 2001. J. Exp. Med. 194:491.\u003cbr\u003e\nGene ID: 7535\u003cbr\u003e\nUniProt: View information about ZAP70 Phospho Tyr292 on UniProt.org\u003cbr\u003e\nClone: A16038B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864393175209,"sku":"693802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-693802","provider":"Iright","version":"1.0","type":"link"}