{"product_id":"biolegend-695502","title":"Biolegend, 695502, Purified anti-BAD Antibody, 100μg","description":"\u003cp\u003eBCL-2 associated agonist of cell death (BAD) is a member of the BCL-2 family, which is involved in the regulation of the mitochondrial apoptosis pathway. The BCL-2 protein family members can either promote cell survival (BCL-2, BCL-XL, and MCL-1) or induce apoptosis (BAX, BAK, BIM, BID, and BAD). The BCL-2 family proteins form homodimers or heterodimers through the conserved BCL-2 homology motifs (BH1, BH2, BH3, and BH4). BAD belongs to the BH3-only proteins and functions as a direct antagonist of the prosurvival effect mediated by BCL-2 and BCL-XL. BAD is inactivated by several survival kinases, such as AKT, PKA, and RSK, through phosphorylation. Upon stimulation of survival signals, phosphorylated BAD dissociates from BCL-2\/ BCL-XL complexes and is sequestered in the cytoplasm by 14-3-3 scaffold proteins. Without interacting with BAD, BCL-2 and BCL-XL are able to suppress apoptosis by preventing release of cytochrome c from mitochondria and blocking subsequent activation of caspases.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full length mouse BAD recombinant protein expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Chea C, et al. 2018. PLoS One. 13:e0191683. PubMed\u003cbr\u003e\nRRID: AB_2650854 (BioLegend Cat. No. 695502)\u003cbr\u003e\nStructure: 204 amino acids with a predicted molecular weight of 22.1 kD. Contains a BH3 domain responsible for dimer formation.\u003cbr\u003e\nDistribution: Mitochondrion outer membrane and cytoplasm.\u003cbr\u003e\nFunction: BAD is a proapoptotic protein, promoting cell apoptosis through inactivating the BCL-2 family members BCL-2 and BCL-XL.\u003cbr\u003e\nInteraction: Forms homodimers or heterodimers with other members of the same family. Interacts with S100A10, 14-3-3, AKT1, PIM3, NOL3, and HIF3A.\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology\u003cbr\u003e\nAntigen References: 1. Cekanova M, et al. 2015. Exp. Cell. Res. 331:1. 2. Pareja F, et al. 2014. Mol. Cancer Res. 12:987. 3. Sakamaki J, et al. 2011. Proc. Natl. Acad. Sci. USA 108:6085. 4. Yu C, et al. 2004. Mol. Cell. 13:329-40. 5. Cartier A, et al. 2003. Exp. Cell Res. 291:242. 6. Moriishi K, et al. 2002. Virology 292:258.\u003cbr\u003e\nGene ID: 12015\u003cbr\u003e\nUniProt: View information about BAD on UniProt.org\u003cbr\u003e\nClone: W16055A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bcl2-associated agonist of cell death, Bcl-2-binding component 6 (Bbc6), Bcl-xL\/Bcl-2-associated death promoter, Bcl2 antagonist of cell death.\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866303090857,"sku":"695502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-695502","provider":"Iright","version":"1.0","type":"link"}